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ceramic fluoroapatite column  (Bio-Rad)


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    Structured Review

    Bio-Rad ceramic fluoroapatite column
    Ceramic Fluoroapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cft+ceramic+fluoroapatite+column/CFT+Ceramic+Fluoroapatite/us12297461-578-26-35
    Average 93 stars, based on 12 article reviews
    ceramic fluoroapatite column - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip ( Tulipa gesneriana ): identification of the third isozyme with a distinct expression profile.
    Article Snippet: The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT ceramic fluoroapatite column (1.0 × 2.5 cm; 2 mL, Bio-Rad, Hercules, CA, USA) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme.
    Article Snippet: The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT Ceramic Fluoroapatite column (0.8 × 4.7 cm, Bio-Rad) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).

    SDS Page:

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip ( Tulipa gesneriana ): identification of the third isozyme with a distinct expression profile.
    Article Snippet: The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT ceramic fluoroapatite column (1.0 × 2.5 cm; 2 mL, Bio-Rad, Hercules, CA, USA) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme.
    Article Snippet: The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT Ceramic Fluoroapatite column (0.8 × 4.7 cm, Bio-Rad) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).

    Staining:

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip ( Tulipa gesneriana ): identification of the third isozyme with a distinct expression profile.
    Article Snippet: The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT ceramic fluoroapatite column (1.0 × 2.5 cm; 2 mL, Bio-Rad, Hercules, CA, USA) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme.
    Article Snippet: The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT Ceramic Fluoroapatite column (0.8 × 4.7 cm, Bio-Rad) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).

    Fractionation:

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip ( Tulipa gesneriana ): identification of the third isozyme with a distinct expression profile.
    Article Snippet: The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (four fractions, 4 mL), concentrated by ultrafiltration with an Amicon Ultra-4 (10 kDa) (Merck Millipore, Billerica, MA, USA), and the buffer was exchanged to 10 mM by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT ceramic fluoroapatite column (1.0 × 2.5 cm; 2 mL, Bio-Rad, Hercules, CA, USA) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (10–500 mM; 10 mL for each buffer) and 0.5-mL fractions were collected.

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip (Tulipa gesneriana): identification of the root-specific isozyme.
    Article Snippet: The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.The active fractions were combined (two fractions, 3.4 mL) and the buffer was exchanged to 5 mM (pH 6.0) by passing through a PD-10 column equilibrated with the same buffer.. The solution was loaded onto a CFT Ceramic Fluoroapatite column (0.8 × 4.7 cm, Bio-Rad) equilibrated with the same buffer.. After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).After washing the column with 10 mL of the same buffer, the column was eluted with a linear gradient of buffer (5–500 mM, pH 6.0; 10 mL for each buffer; 0.4 mL fractions).



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    Figure 3. Purification of TCEB from tulip leaves. Protein from each purification step was separated on 10% SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. Lane M, Molecular size markers; lane 1, crude extract; lane 2, (NH4)2SO4 fractionation; lane 3, DIAION-HPA25L fractions; lane 4, DEAE-Toyopearl fractions; lane 5, <t>CFT-ceramic</t> <t>fluoroapatite</t> fractions; lane 6, Superdex 200 (1st) fractions; lane 7, Superdex 200 (2nd) fractions.
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    Image Search Results


    Figure 3. Purification of TCEB from tulip leaves. Protein from each purification step was separated on 10% SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. Lane M, Molecular size markers; lane 1, crude extract; lane 2, (NH4)2SO4 fractionation; lane 3, DIAION-HPA25L fractions; lane 4, DEAE-Toyopearl fractions; lane 5, CFT-ceramic fluoroapatite fractions; lane 6, Superdex 200 (1st) fractions; lane 7, Superdex 200 (2nd) fractions.

    Journal: Bioscience, biotechnology, and biochemistry

    Article Title: Molecular diversity of tuliposide B-converting enzyme in tulip ( Tulipa gesneriana ): identification of the third isozyme with a distinct expression profile.

    doi: 10.1080/09168451.2018.1438170

    Figure Lengend Snippet: Figure 3. Purification of TCEB from tulip leaves. Protein from each purification step was separated on 10% SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. Lane M, Molecular size markers; lane 1, crude extract; lane 2, (NH4)2SO4 fractionation; lane 3, DIAION-HPA25L fractions; lane 4, DEAE-Toyopearl fractions; lane 5, CFT-ceramic fluoroapatite fractions; lane 6, Superdex 200 (1st) fractions; lane 7, Superdex 200 (2nd) fractions.

    Article Snippet: The solution was loaded onto a CFT ceramic fluoroapatite column (1.0 × 2.5 cm; 2 mL, Bio-Rad, Hercules, CA, USA) equilibrated with the same buffer.

    Techniques: Purification, SDS Page, Staining, Fractionation